Fig 5. US27 colocalizes with CXCR4 after CXCL12 treatment but also internalizes independently.

3T3-CXCR4/US27 cells were seeded in glass-bottom dishes, labeled with 100nM membrane impermeable αRED (CXCR4) and βGREEN (US27) fluorogens, and then treated with (A) PBS or (B) 100 ng/ml CXCL12. Images were acquired every five minutes with representative images shown. Scale bar, 20 μm. White arrows indicate discrete yellow punctae indicating overlapping red and green signal.