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. 2017 Feb 16;12(2):e0172445. doi: 10.1371/journal.pone.0172445

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© 2017 Regenthal et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 1 — (A, C, E) Size-exclusion chromatography of SEA, SEE and SEH, with protein purities analyzed by coomassie-stained SDS-PAGE gels shown as insets. (B, D, F) Far-UV CD spectra for SEA, SEE, SEH at 200–250 nm at 20°C in presence of 0.1 mM ZnCl₂ (dark grey) or 1 mM EDTA (light grey) at pH 6.0.