Table 1. PCR primers for real time PCR assay with amplicon size and annealing temperature.

Target organism	Primer sequence (Forward/Reverse)	Size (bp)	Anneal temp. (°C)	Reference
Bacteria	F-5’CGG CAA CGA GCG CAA CCC-3’F-5’CCA TTG TAG CAC GTG TGT AGC C-3’	130	60	[19]
Fungi	F- 5’GAG GAA GTA AAA GTC GTA ACA AGG TTT -3’R-5’CCA AAT TCA CAA AGG GTA GGA TGAT T-3’	110	60	[19]
Methanogen	F 5’-TTC GGT GGA TCD CAR AGR GC-3’R 5’-GBA RGT CGW AWC CGT AGA ATC C-3’	140	60	[20]
Ruminococcus albus	F-5’CCCTAAACAGTCTTAGTTCG-3’R-5’CCT CCT TGC GGT TAG AAC A-3’	175	60	[21]
R. flavefaciens	F-5’CGA ACG GAG ATA ATT TGA GTT TAC TTA G-3’R-5’CGG TCT CTG TAT GTT ATG AGG TAT TA-3’	132	60	[19]
Fibrobacter succinogenes	F-5’GTT CGG AAT TAC TGG GCG TAA A-3’R-5’CGC CTG CCC CTG AAC TATC-3’	121	60	[19]