Quantitative RT-PCR was performed on whole mouse kidney total RNA after 9 weeks following diabetes. Fibronectin (A), and type-1 collagen (B) mRNA expression were normalized with GAPDH mRNA. Open bar, control groups; black-filled bar, diabetic groups. Results are means ± SEM. *p<0.05, **p<0.01 compared to control. #p<0.05, ##p<0.01 compared to diabetic Ccr2−/− mice.