Fig. 2.

a Restriction map of vector used to transform North-East rice cultivars and IR 64. b–d The regeneration of plantlets from putative transgenic calli on MS-REM medium from IR 64, Khongan and Taotabhi, respectively. Southern blot hybridization to confirm stable integration of transgenes into genomic DNA of Khongan (e), Taothabi (f) and IR 64 (g). Blots were hybridized with the coding region of hpt gene. Note the presence of different copies of hpt gene in different transgenic lines which is common when transformed with Agrobacterium mediated method. h Northern blot analysis to confirm the transcription of hpt gene in randomly chosen ten transgenic IR 64 plants. M Marker DNA in kbs, C control untransformed plant, 1–10 transgenic plants