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Effects of pre-cultured seed duration on Agrobacterium-mediated transformation in rice cv. IR64
| No. of days seeds were pre-cultured before infection with Agrobacterium | No. of co-cultured seeds | Percentage of co-cultured seeds that produced hygromycin resistant callia | Percentage of hygromycin resistant calli that are also positive for GFP expressiona |
|---|---|---|---|
| 1 | 140 | 20.0 | 12.1 |
| 2 | 130 | 26.2 | 16.9 |
| 3 | 120 | 31.7 | 30.8 |
| 4 | 110 | 38.2 | 37.3 |
| 5 | 110 | 50.9 | 45.5 |
aAfter 14 days of culture on selection medium
