Table 2.

List of primer pairs and polymerase chain reaction (PCR) annealing temperatures of candidate barcodes markers used

Locus	Primer name	Sequence	Annealing temperature	References
rbcL	rbcL 1F	ATGTCACCACAAACAGAAAC	46	Fay et al. (1998)
	rbcL 724R	TCGCATGTACCTGCAGTAGC
	rbcL aF	ATGTCACCACAAACAGAGACTAAAGC	48	Levin et al. (2003), Kress et al. (2009)
	rbcL aR	GTAAAATCAAGTCCACCRCG
matK	mat K 390	CGATCTATTCATTCAATATTC	48	Cuénoud et al. (2002)
	matK 1326	TCTAGCACACGAAAGTCGAAGT
	matK F	ACCCAGTCCATCTGGAAATCTTGGTTC	50	Fofana et al. (1997)
	matK R	CGTACAGTACTTTTGTGTTTACGAG
ITS	ITS 8F	AACAAGGTTTCCGTAGGTGA	58	Wen and Zimmer (1996)
	ITS 9R	TATGCTTAAAYTCAGCGGGT
	ITS 10F	CGAACACGTTACAATACCG	55
	ITS 11R	ACCACTTGTCGTGACGTCC
trnLC–trnLD	trnL C	CGAAATCGGTAGACGCTACG	48.5	Taberlet et al. (1991)
	trnL D	GGGGATAGAGGGACTTGAAC
trnH–psbA	psbA	GTTATGCATGAACGTAATGCTC	55	Sang et al. (1997)
	trnH	CGCGCATGGTGGATTCACAATCC		Tate and Simpson (2003)