Table 4.
RAPD and ISSR primers and their characteristics
| No | Name | Annealing temperature used (Ta) | No. of Bands | Band size (bp) | No. of polymorphic bands | % polymorphism |
|---|---|---|---|---|---|---|
| RAPD | ||||||
| 1 | RPi 1 | 38.0 | 22 | 119–1507 | 18 | 81.18 |
| 2 | RPi 2 | 39.0 | 20 | 85–660 | 17 | 85.00 |
| 3 | RPi 3 | 38.5 | 21 | 85–117 | 17 | 80.95 |
| 4 | RPi 4 | 38.0 | 23 | 313–1082 | 18 | 78.36 |
| 5 | RPi 5 | 40.0 | 16 | 530–1340 | 13 | 81.25 |
| 6 | RPi 6 | 39.0 | 17 | 450–920 | 11 | 64.79 |
| 7 | RPi 7 | 40.0 | 13 | 480–1340 | 10 | 76.92 |
| 8 | RPi 9 | 38.5 | 14 | 77–682 | 11 | 78.57 |
| 9 | RPi 10 | 38.0 | 16 | 80–387 | 11 | 68.75 |
| ISSR | ||||||
| 1 | UBC 2 | 44.6 | 11 | 170–2900 | 9 | 81.81 |
| 2 | UBC 3 | 44.6 | 14 | 340–2050 | 8 | 57.14 |
| 3 | UBC 4 | 44.6 | 8 | 300–1950 | 7 | 87.50 |
| 4 | UBC 5 | 49.2 | 10 | 450–2370 | 8 | 80.00 |
| 5 | UBC 6 | 49.2 | 9 | 250–3010 | 7 | 77.77 |
| 6 | UBC 18 | 59.8 | 12 | 300–1810 | 9 | 75.00 |
| 7 | UBC 22 | 55.0 | 13 | 1060–2240 | 10 | 76.92 |
| 8 | UBC 26 | 60.0 | 13 | 200–3200 | 11 | 84.61 |
| 9 | UBC 27 | 60.0 | 9 | 600–1790 | 6 | 66.66 |
| 10 | UBC 36 | 59.8 | 14 | 470–2740 | 11 | 78.57 |