Table 3.
Activity of superoxide dismutase, catalase, ascorbate peroxidase and glutathione reductase in flag leaves of Aegilops tauschii, Triticum dicoccoides and cultivated wheats under irrigated and rain-fed conditions
| Species | Accessions | Superoxide dismutase | Catalase | Ascorbate peroxidase | Glutathione reductase | ||||
|---|---|---|---|---|---|---|---|---|---|
| Irrigated | Rain-fed | Irrigated | Rain-fed | Irrigated | Rain-fed | Irrigated | Rain-fed | ||
| MEAN ± S.D. | MEAN ± S.D. | MEAN ± S.D. | MEAN ± S.D. | MEAN ± S.D. | MEAN ± S.D. | MEAN ± S.D. | MEAN ± S.D. | ||
| Aegilops tauschii (diploid) | 3769 | 16.89 ± 5.64 | 31.48 ± 2.06 | 3007.45 ± 240.66 | 4264.26 ± 581.97 | 7.66 ± 0.35 | 13.19 ± 2.49 | 1495.18 ± 68.21 | 2218.65 ± 30.32 |
| (0.39 ± 0.07) | (0.65 ± 0.02) | (70.87 ± 5.01) | (87.85 ± 9.52) | (0.182 ± 0.04) | (0.272 ± 0.04) | (35.57 ± 6.94) | (45.80 ± 1.92) | ||
| bcdef | a (186.38%) | efgh | a (141.79%) | ij | cdef (172.19%) | defghi | ab (148.39%) | ||
| 14189 | 17.70 ± 4.50 | 12.57 ± 1.93 | 2397.53 ± 192.30 | 3698.87 ± 516.75 | 4.39 ± 1.05 | 11.35 ± 3.86 | 1085.21 ± 102.31 | 2067.26 ± 316.42 | |
| (0.42 ± 0.17) | (0.27 ± 0.02) | (55.63 ± 13.92) | (79.37 ± 4.87) | (0.103 ± 0.04) | (0.242 ± 0.06) | (24.80 ± 1.94) | (44.33 ± 3.32) | ||
| bcdef | defgh (71.02%) | hi | abcde (154.28%) | k | efgh (258.54%) | hijk | bc (190.49%) | ||
| 9809 | 10.38 ± 3.14 | 19.38 ± 6.82 | 2905.66 ± 283.39 | 2929.12 ± 429.03 | 5.81 ± 1.13 | 12.89 ± 2.05 | 699.36 ± 34.10 | 1188.37 ± 252.00 | |
| (0.15 ± 0.02) | (0.54 ± 0.10) | (42.62 ± 2.55) | (83.47 ± 1.81) | (0.087 ± 0.03) | (0.367 ± 0.01) | (10.38 ± 2.13) | (33.68 ± 1.51) | ||
| gh | bc (186.71%) | fghi | fghi (100.81%) | jk | cdef (221.86%) | k | fghijk (169.92%) | ||
| 14130 | 16.57 ± 0.67 | 19.20 ± 4.81 | 2903.67 ± 225.48 | 3286.16 ± 92.78 | 6.19 ± 0.69 | 5.95 ± 2.43 | 1542.07 ± 202.73 | 1346.47 ± 28.42 | |
| (0.25 ± 0.01) | (0.30 ± 0.09) | (44.33 ± 2.95) | (51.49 ± 0.45) | (0.095 ± 0.01) | (0.22 ± 0.05) | (23.57 ± 3.36) | (21.12 ± 1.23) | ||
| bcdefg | bc (115.87%) | fghi | cdefg (113.17%) | jk | jk (96.12%) | defghi | efghi (87.32%) | ||
| 14096 | 21.49 ± 0.86 | 29.55 ± 4.53 | 2505.68 ± 140.57 | 3307.63 ± 503.25 | 6.63 ± 0.52 | 8.31 ± 1.86 | 1574.23 ± 229.26 | 2244.11 ± 403.57 | |
| (0.55 ± 0.01) | (0.73 ± 0.03) | (64.25 ± 6.87) | (82.22 ± 2.93) | (0.170 ± 0.04) | (0.214 ± 0.09) | (40.46 ± 7.92) | (57.54 ± 20.77) | ||
| b | a (137.51%) | hi | cdefg (132.01%) | jk | hij (125.34%) | cdefgh | ab (142.55%) | ||
| 14113 | 16.34 ± 3.71 | 29.37 ± 0.13 | 2286.60 ± 652.82 | 3757.32 ± 50.61 | 8.64 ± 0.19 | 8.55 ± 0.81 | 1228.94 ± 636.31 | 2676.85 ± 306.94 | |
| (0.36 ± 0.07) | (0.54 ± 0.01) | (50.98 ± 13.33) | (69.46 ± 0.75) | (0.193 ± 0.02) | (0.158 ± 0.01) | 35.61 ± 10.53 | (49.49 ± 5.80) | ||
| bcdefg | a (179.74%) | i | abc (164.32%) | hij | hij (98.96%) | fghij | a (217.82%) | ||
| 14219 | 13.71 ± 1.14 | 12.27 ± 1.24 | 2241.67 ± 291.27 | 4073.41 ± 32.05 | 13.97 ± 0.56 | 12.61 ± 3.48 | 819.94 ± 477.47 | 1944.00 ± 20.84 | |
| (0.21 ± 0.03) | (0.28 ± 0.12) | (35.03 ± 6.93) | (90.61 ± 30.37) | (0.218 ± 0.02) | (0.293 ± 0.17) | (12.50 ± 6.55) | (43.26 ± 14.62) | ||
| cdefgh | efgh (89.5%) | i | ab (181.71%) | cde | defg (90.26%) | jk | bcd (237.09%) | ||
| 14129 | 14.43 ± 1.01 | 19.24 ± 5.03 | 3301.27 ± 405.41 | 3592.32 ± 581.97 | 5.99 ± 0.56 | 6.48 ± 0.71 | 1350.48 ± 151.58 | 2072.62 ± 20.84 | |
| (0.30 ± 0.03) | (0.32 ± 0.05) | (67.88 ± 9.34) | (59.78 ± 3.56) | (0.123 ± 0.01) | (0.108 ± 0.01) | (27.72 ± 2.70) | (34.76 ± 3.23) | ||
| cdefgh | bc (133.33%) | cdefg | abcdef (108.82%) | jk | jk (108.18%) | efghi | bc (153.47%) | ||
| 14231 | 18.74 ± 7.10 | 16.51 ± 4.94 | 2856.36 ± 292.39 | 3572.04 ± 58.48 | 6.12 ± 1.08 | 10.72 ± 1.75 | 1116.03 ± 418.73 | 1619.78 ± 835.57 | |
| (0.28 ± 0.06) | (0.28 ± 0.08) | (43.31 ± 2.23) | (60.39 ± 1.71) | (0.095 ± 0.03) | (0.54 ± 0.08) | (17.55 ± 9.02) | (27.30 ± 13.80) | ||
| bcd | bcdefg (88.10%) | ghi | abcdef (125.06%) | jk | fgh (175.16%) | ghijk | cdefg (145.14%) | ||
| Triticum dicoccoides (tetraploid) | 7054 | 14.55 ± 3.78 | 18.42 ± 1.68 | 4201.84 ± 213.67 | 4082.56 ± 516.18 | 15.47 ± 1.88 | 17.77 ± 3.05 | 818.60 ± 66.32 | 1070.47 ± 96.63 |
| (0.34 ± 0.09) | (0.41 ± 0.03) | (98.90 ± 6.63) | (90.23 ± 9.16) | (0.364 ± 0.05) | (0.392 ± 0.06) | (19.27 ± 1.87) | (23.72 ± 2.73) | ||
| cdefgh | bcde (126.60%) | a | ab (97.16%) | bcd | ab (114.87%) | jk | hijk (130.77%) | ||
| 7079 | 9.50 ± 0.71 | 13.76 ± 4.32 | 3596.29 ± 581.97 | 3253.96 ± 829.94 | 18.72 ± 1.75 | 15.09 ± 4.46 | 1828.78 ± 483.15 | 1532.69 ± 49.26 | |
| (0.24 ± 0.03) | (0.46 ± 0.30) | (90.85 ± 11.48) | (97.39 ± 12.36) | (0.473 ± 0.03) | (0.448 ± 0.04) | (46.11 ± 10.58) | (47.89 ± 16.56) | ||
| h | cdefgh (144.84%) | abcdef | cdefg (90.48%) | a | bcd (80.61%) | bcde | defghi (83.81%) | ||
| 14004 | 11.68 ± 5.44 | 9.84 ± 2.27 | 3752.55 ± 237.29 | 3023.75 ± 241.22 | 15.70 ± 1.39 | 18.03 ± 1.40 | 1321.01 ± 64.42 | 1463.03 ± 49.26 | |
| (0.27 ± 0.09) | (0.30 ± 0.06) | (89.49 ± 6.75) | (94.38 ± 12.01) | (0.374 ± 0.02) | (0.561 ± 0.02) | (31.54 ± 2.83) | (45.54 ± 0.64) | ||
| fgh | h (84.25%) | abc | defgh (80.58%) | abc | ab (114.84%) | efghij | defghi (110.75%) | ||
| Cultivated checks | C-306 | 22.16 ± 3.59 | 11.87 ± 0.38 | 4123.91 ± 919.91 | 3478.20 ± 229.41 | 8.39 ± 0.39 | 9.69 ± 1.34 | 1334.41 ± 128.84 | 1893.09 ± 449.05 |
| (hexaploids) | (0.48 ± 0.01) | (0.28 ± 0.08) | (88.36 ± 5.59) | (83.17 ± 25.66) | (0.182 ± 0.02) | (0.234 ± 0.09) | (28.89 ± 1.87) | (43.60 ± 0.35) | |
| b | fgh (53.56%) | ab | bcdefg (84.34%) | hij | ghi (115.49%) | efghij | bcd (141.87%) | ||
| PBW-343 | 19.03 ± 4.16 | 18.70 ± 7.07 | 3940.61 ± 372.80 | 3721.54 ± 489.54 | 6.28 ± 0.71 | 17.81 ± 2.22 | 1028.94 ± 606.31 | 1647.91 ± 170.52 | |
| (0.58 ± 0.21) | (0.59 ± 0.29) | (117.88 ± 6.91) | (116.19 ± 30.14) | (0.188 ± 0.01) | (0.547 ± 0.00) | (29.61 ± 13.53) | (50.67 ± 1.36) | ||
| bc | bcd (98.27%) | abc | abcd (94.44%) | jk | ab (283.6%) | ijk | cdef (160.16%) | ||
Accessions have been arranged in descending order of ranking (based on cumulative tolerance index) in each respective ploidy level
Values are mean ± S.D. of three replicates. Value without parenthesis is the activity of the respective enzyme, whereas value within the parenthesis is the specific activity of that enzyme. Percentages in bold represents change in the activity of the respective enzymes under rain-fed conditions (keeping base as 100 for irrigated conditions). Values with same alphabet(s) are not significantly different at P ≤ 0.05 (Fisher’s LSD test)
Superoxide dismutase activity is represented as units min-1 g-1 FW and specific activity as units min-1 mg-1 protein.
A unit of catalase activity is represented as µmole of H2O2 decomposed min-1 g-1 FW and specific activity as µmole of H2O2 decomposed min-1 mg-1 protein
A unit of ascorbate peroxidase activity is represented as µmole of monodehydroascorbate formed min-1 g-1FW and specific activity as µmole of monodehydroascorbate formed min-1 mg-1 protein.
A unit of glutathione reductase activity is represented as µmole of NADP+ formed min-1 g-1 FW and specific activity as µmole of NADP+ formed min-1 mg-1 protein