Table 2.
Differentially abundant proteins and their identification by MALDI-TOF/TOF-MS in B-toxic Citrus sinensis roots.
| Spot No. | Protein identity | Accession no. | Mr(kDa)/pI Theor. | Mr (kDa)/pI Exp. | Reference species | Protein score | NMP | Ratio | Covered sequence (%) | Charge |
|---|---|---|---|---|---|---|---|---|---|---|
| STRESS RESPONSE | ||||||||||
| S16 | Heat shock protein 83 | gi|169296 | 80.77/4.95 | 81.1/5.0 | Ipomoea nil | 102 | 8 | 0.42 ± 0.20 | 14 | 1 |
| S32 | Mitochondrial chaperonin hsp60 | gi|20466256 | 61.242/5.66 | 60.3/5.18 | Arabidopsis thaliana | 288 | 22 | 1.92 ± 0.31 | 29 | 1 |
| S17 | Late-embryogenesis abundant protein 2 | gi|212552206 | 34.343/4.72 | 35.2/4.53 | Glycine max | 260 | 14 | 1.87 ± 0.31 | 36 | 1 |
| CELL WALL AND CYTOSKELETON | ||||||||||
| S30 | Actin | gi|6103623 | 41.564/5.30 | 42.6/5.81 | Picea rubens | 637 | 25 | 1.63 ± 0.10 | 50 | 1 |
| S29 | Alpha-tubulin | gi|321437427 | 46.368/4.90 | 46.2/4.8 | Musa acuminata AAA Group | 647 | 21 | 1.70 ± 0.26 | 47 | 1 |
| S18 | Beta-tubulin 14 | gi|166343835 | 49.98/4.76 | 50.4/4.6 | Gossypium hirsutum | 618 | 31 | 2.97 ± 0.20 | 40 | 1 |
| S19 | Tubulin β-1 chain | gi|332197637 | 50.185/4.68 | 51.6/4.91 | Arabidopsis thaliana | 660 | 27 | 2.19 ± 0.46 | 33 | 1 |
| S3 | Profilin | gi|12659206 | 14.133/4.90 | 13.8/4.94 | Corylus avellana | 89 | 9 | 1.57 ± 0.04 | 43 | 1 |
| CARBOHYDRATE AND ENERGY METABOLISM | ||||||||||
| S27 | Adenosine kinase 2, partial | gi|149391003 | 26.477/4.88 | 25.9/5.63 | Oryza sativa Indica Group | 80 | 7 | 1.82 ± 0.15 | 21 | 1 |
| S28 | Adenosine kinase isoform 1T-like protein | gi|82400168 | 37.549/5.01 | 38.6/5.71 | Solanum tuberosum | 113 | 6 | 1.81 ± 0.14 | 20 | 1 |
| S47 | Malate dehydrogenase, partial | gi|160690776 | 14.017/7.03 | 15.4/5.05 | Citrus trifoliata | 220 | 6 | 0.50 ± 0.09 | 38 | 1 |
| PROTEIN AND AMINO ACID METABOLISM | ||||||||||
| S9 | Proteasome subunit alpha type, putative | gi|255584432 | 27.0/4.73 | 27.8/4.75 | Ricinus communis | 191 | 11 | 1.76 ± 0.12 | 42 | 1 |
| S12 | Elongation factor 1-delta 1 | gi|226505926 | 24.767/4.39 | 23.8/4.38 | Zea mays | 219 | 11 | 2.41 ± 0.31 | 20 | 1 |
| S15 | Elongation factor 2 | gi|195646972 | 93.862/6.00 | 94.2/6.51 | Zea mays | 143 | 8 | 2.90 ± 0.21 | 9 | 1 |
| S23 | Translation initiation factor | gi|197312901 | 16.419/4.98 | 16.2/5.12 | Rheum australe | 188 | 7 | 1.72 ± 0.08 | 26 | 1 |
| S40 | Eukaryotic translation initiation factor 2 beta subunit-like | gi|82621136 | 29.831/6.08 | 30.6/6.54 | Solanum tuberosum | 101 | 9 | 1.85 ± 0.15 | 32 | 1 |
| S36 | Eukaryotic translation initiation factor 5A1 | gi|217038830 | 17.387/5.60 | 17.2/5.7 | Glycine max | 153 | 10 | 3.13 ± 0.72 | 28 | 1 |
| S34 | Eukaryotic initiation factor 5A (2) | gi|19702 | 17.353/5.60 | 18.5/6.4 | Nicotiana plumbaginifolia | 120 | 10 | 0.48 ± 0.09 | 58 | 1 |
| S8 | Alpha chain of nascent polypeptide associated complex | gi|124484511 | 21.911/4.32 | 22.1/4.52 | Nicotiana benthamiana | 242 | 8 | 2.03 ± 0.37 | 20 | 1 |
| S43 | Alanine aminotransferase 2 | gi|332197185 | 47.68/6.32 | 48.2/6.61 | Arabidopsis thaliana | 138 | 5 | 2.28 ± 0.30 | 11 | 1 |
| S41 | S-adenosylmethionine synthase 2 | gi|1655578 | 42.977/5.51 | 43.3/5.93 | Catharanthus roseus | 241 | 19 | 1.89 ± 0.14 | 47 | 1 |
| S39 | 5-methyltetrahy dropteroyltriglutamate-homocysteine methyltransferase, putative | gi|255569484 | 84.668/6.09 | 85.6/6.52 | Ricinus communis | 373 | 19 | 1.97 ± 0.16 | 27 | 1 |
| S42 | Transaminase mtnE, putative | gi|255562088 | 50.396/6.95 | 51.8/6.05 | Ricinus communis | 244 | 10 | 1.74 ± 0.16 | 22 | 1 |
| S31 | Ketol-acid reductoisomerase | gi|295291644 | 63.583/6.49 | 64.6/6.62 | Catharanthus roseus | 286 | 16 | 2.07 ± 0.38 | 21 | 1 |
| NUCLEIC ACID METABOLISM | ||||||||||
| S13 | Glycine-rich RNA-binding protein 4 | gi|332643299 | 14.12/5.03 | 15.6/8.68 | Arabidopsis thaliana | 133 | 4 | 1.85 ± 0.13 | 33 | 1 |
| S21 | Glycine-rich RNA-binding protein | gi|7024451 | 16.839/4.98 | 17.4/7.85 | Citrus unshiu | 227 | 10 | 1.92 ± 0.31 | 28 | 1 |
| CELLULAR TRANSPORT | ||||||||||
| S20 | Vacuolar H+-ATPase B subunit | gi|4519264 | 54.329/4.91 | 55.6/4.91 | Citrus unshiu | 641 | 35 | 1.67 ± 0.15 | 53 | 1 |
| BIOLOGICAL REGULATION AND SIGNAL TRANSDUCTION | ||||||||||
| S44 | Nucleoside diphosphate kinase 1 | gi|255571035 | 176.41/5.09 | 179.1/6.3 | Ricinus communis | 136 | 4 | 1.51 ± 0.16 | 20 | 1 |
| S14 | 14-3-3 family protein | gi|291162645 | 29.404/4.74 | 30.5/4.71 | Dimocarpus longan | 241 | 7 | 1.52 ± 0.14 | 33 | 1 |
| S6 | Translationally controlled tumor-like protein | gi|115187479 | 19.116/4.54 | 20.0/4.7 | Arachis hypogaea | 286 | 9 | 0.44 ± 0.14 | 31 | 1 |
| OTHER | ||||||||||
| S37 | Unnamed protein product, partial | gi|296088008 | 17.163/5.21 | 18.2/7.93 | Vitis vinifera | 709 | 17 | 1.84 ± 0.27 | 51 | 1 |
Spot number corresponds to the 2-DE gel imagines in Figures 2A,B. NMP means the number of peptides. Ratio means the ratio of B-toxic roots to controls and the values were the means ± SE of three replicates. Covered sequence (%) means the ratio of the number of amino acids of the matched peptides to the number of amino acids of the full-length protein. Proteins shared by C. sinensis and C. grandis roots were marked in bold.