Table 1.
Cytokine production and the proliferative responses of splenocytes from BALB/c mice immunized with PBS, pVAX1, pVAX1-TgSPATR, or blank control.
| Groups (n = 5) | Cytokine production (pg/ml)a | Proliferation(SI)b | |||
|---|---|---|---|---|---|
| IFN-γ | IL-2 | IL-4 | IL-10 | ||
| Blank control | 44.23 ± 1.92 | 45.80 ± 1.73 | 48.99 ± 0.79 | 48.54 ± 0.12 | 0.64 ± 0.05 |
| PBS | 47.28 ± 2.34 | 43.43 ± 2.31 | 48.98 ± 3.28 | 49.36 ± 0.11 | 0.62 ± 0.01 |
| pVAX1 | 44.11 ± 1.82 | 46.32 ± 3.26 | 49.19 ± 1.30 | 49.08 ± 0.03 | 0.55 ± 0.02 |
| pVAX1-TgSPATR | 672.87 ± 8.35* | 367.93 ± 10.30* | 212 ± 7.42* | 261.8 ± 10.03* | 1.24 ± 0.14* |
a
Values for IL-2, IL-4, IL-10 and IFN- γ are for 24, 24, 72, and 96 h, respectively.
b
SI stands for stimulation index. Splenocytes from mice were harvested 2 weeks after the last immunization. Data are presented as the mean ± S.D. (n = 5). Three independent experiments were performed.
*
p < 0.05 compared with the control groups.