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Variable RNA input and cDNA dilutions used for different sample sets
| Sample set | Sample input (μL) | RNA input (μL) | cDNA dilution | LODa (log copies/μL plasma) |
|---|---|---|---|---|
| KCCR | 200 | 5 | 1/50 | 1.64 |
| KCCR | 200 | 5 | 1/8 | 0.85 |
| FR3 + TS | 25 | 6 | 1/50 | 2.46 |
KCCR Kumasi Centre for Collaborative Research, FR3 Filariasis Research Reagent Resource Center, TS Tissue Solutions, Ltd.
aBased on 28 copies/qPCR reaction and 100% extraction and cDNA synthesis efficiency
