Figure 6.

BEX3 knockdown sensitized NPC to cisplatin. (A) QPCR analysis of mRNA expression level of BEX3 in HONE1 with BEX3‐shRNA. (B, C) Colony formation of BEX3‐knockdown HONE1 cells after exposure to 1.0 μmol/L cisplatin. (D, E) Real‐time cell kinetic analysis of cisplatin cytotoxicity using xCELLigence RTCA. Cisplatin was added to BEX3‐knockdown HONE1 cells and cell index was continuously monitored. The IC ₅₀ value was calculated from the dose–response curve after cisplatin treatment for 48 h. Error bar indicate SD, n = 3, **P < 0.01. BEX3, Brain‐expressed X‐linked 3; ICC, Immunocytochemistry; NPC, Nasopharyngeal carcinoma; QPCR, Real‐time quantitative polymerase chain reaction.