TABLE 1.
Results of initial attempts to produce transgenic mice with brain expression of Neu5Gc
Cmah cDNA was expressed under CMV promoter or NSE promoter. With the NSE promoter, a frameshift mutant Cmah cDNA was also tried. The numbers of live births and transgene-positive mice were indicated.
| Promoter | Cmah cDNA | Live births | Founders (transgene-positive) | Transmitted to F1 | Message expression in braina | Increased Neu5Gc in brain |
|---|---|---|---|---|---|---|
| CMV | Wild type (active) | 105 | 4 | 4 | No | No |
| NSE | Wild type (active) | 72 | 2 | 2 | No | No |
| NSE | Mutantb (inactive) | 57 | 9 | 6 | Yes (5) | No |
a The endogenous message is positive in liver.
b Single base pair frameshift mutation is in coding sequence of Cmah.