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. 2016 Dec 27;292(7):2637–2645. doi: 10.1074/jbc.M116.751008

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FIGURE 2. — A 7-bp pre-melted (mismatch) bubble fails to produce promoter-specific transcription. A, graphic representation of the two templates used for in vitro transcription. LWT is composed of two complementary oligonucleotides, whereas LP1 contains a 7-bp mismatch region. B, in vitro transcription from duplex and mismatch oligonucleotide templates as described under “Experimental Procedures.” Reactions with duplex LSP template (LWT, lanes 1–4) or LSP 7-bp mismatch template (LP1, mismatch −3 to +4, lanes 5–8) are shown; complete oligonucleotide sequences are found under “Experimental Procedures.” POLRMT was added to all reactions, and TFAM and TFB2M were added as indicated. The expected LSP run-off products of 19 and 20 nt are indicated. MW, molecular weight marker.