FIGURE 2.

JDP2 diminishes GNRH induction of c-Jun and FSHβ. A, four tandem copies of the CRE element (TGACGTCA) were linked to the minimal heterologous promoter and luciferase reporter in pGL3 backbone and transfected into LβT2 cells with the herpesvirus thymidine kinase-driven β-galactosidase gene as an internal control for transfection efficiency; additionally, cells were co-transfected with empty vector control (ctrl) or expression vector for ATF3 or JDP2. After overnight starvation, cells were treated with vehicle or 10 nm GNRH for 5 h, after which the luciferase and β-galactosidase values were obtained. B and C, c-JUN luciferase plasmid (−1000 bp) was used as a reporter. D, four copies of TRE element (TGAGTCA) served as a reporter. E and F, −1000 bp FSHβ luciferase was transfected as a reporter. *, significant change (p < 0.05) in -fold induction by GNRH, where the GNRH-treated luciferase/β-galactosidase ratio was normalized to vehicle-treated samples transfected with the same expression vector. #, significantly lower luciferase expression from the empty vector control (vehicle-treated samples transfected with overexpression vector compared with vehicle-treated empty vector, and GNRH-treated samples transfected with overexpression compared with GNRH-treated vector control). White bars, vehicle; black bars, GNRH-treated. Error bars, S.E.