FIGURE 6.

Amino acid starvation induces a transient association of Gcn2 with TORC1. A, wild type cells expressing KOG1-HA (Y1599) alone or together with GCN2-myc (Y1603) were starved for histidine. B, wild type cells expressing TOR1-HA (Y1666) alone or together with GCN2-myc (Y1664) were starved for histidine. C, wild type cells expressing AVO1-HA (Y1659) alone or together with GCN2-myc (Y1658) were starved for histidine. Cells were collected at the indicated times after the onset of starvation. Cell extracts were precipitated with control IgG (C2) or Myc antibody. Levels of the tagged proteins in cell extracts and precipitates (IP) were analyzed by Western blotting. The cells without GCN2-myc expression were used as a negative control (C1). The experiments were repeated three times, and representative data are shown.