TABLE 2.
Plasmids used in this study
| Plasmids | Characteristics | Source/Reference |
|---|---|---|
| PB115 | gcn2 (K628R) in pRS316 (CEN URA3) | Ref. 44 |
| pDH101 | GCN2-FLAG in pRS316 (CEN URA3) | Ref. 44 |
| pDH109 | pGAL1-gcn2 (K628R)-FLAG in pEMBLyex4 (2μ URA3 leu2-d) | Ref. 7 |
| pDH114 | pGAL1-GCN2 (E803V)-FLAG in pEMBLyex4 (2μ URA3 leu2-d) | Ref. 7 |
| pJB948 | kog1Δ5-3HA in pRS406 (URA3) | Ref. 26 |
| pJB1321 | SCH9-3HA in Ycplace33 (CEN URA3) | Ref. 29 |
| pJB1379 | 3HA-tor1Δ3 in pRS406 (URA3) | Laboratory stock |
| pJB1404 | kog1Δ5-13myc in pRS406 | Ref. 26 |
| pJB1405 | GFP-ATG8-pRS416 (CEN URA3) | Ref. 48 |
| pJB1421 | gcn2Δ5-13myc-ADH1 terminator in pRS406 (CEN URA3) | This study |
| pJB1440 | GFP-ATG8-pRS414 (CEN TRP1) | This study |
| pJB1574 | GCN2 (E803V)-FLAG in pRS316 (CEN URA3) | This study |
| pJB1575 | gcn2 (K628R-FLAG in pRS316 (CEN URA3) | |
| pJB1580 | SCH9-3HA in pRS415 (CEN LEU2) | This study |
| pJM186 | GTR2 (S23L) in pRS414 (CEN TRP1) | This study |
| pJU654 | GTR2 (S23L) in pRS416 (CEN URA3) | Ref. 25 |
| pMB1483 | GTR1 (Q65L) in pRS415 (CEN LEU2) | Ref. 25 |