FIGURE 4.

Ulk1 suppressed the stability of Cdc37's clients by inducing its phosphorylation. A, Ulk1-KO HCT116 cells were transfected with the indicated plasmids in the presence or absence of MG132. 24 h after transfection proteins were extracted for Western blotting. B, HCT116 cells were transfected with the indicated plasmids. 24 h after transfection proteins were extracted for Western blotting (IB). IP, immunoprecipitation. C, HCT116 cells were transfected with the indicated plasmids. 24 h after transfection cells were treated with phenformin for another 24 h. Proteins were then extracted for Western blotting.