FIGURE 5.
In vivo aminoacylation assays. A, 2 μg of total mitochondrial RNAs purified from eight cell lines under acid conditions were electrophoresed at 4 °C through an acid (pH 5.2) 10% polyacrylamide with 7 m urea gel, electroblotted, and hybridized with a DIG-labeled oligonucleotide probe-specific for the tRNALys, tRNATyr, tRNALeu(CUN), and tRNASer(AGY), respectively. B, in vivo aminoacylated proportions of tRNALys, tRNATyr, tRNALeu(CUN), and tRNASer(AGY) in the mutant and controls. The calculations were based on three independent determinations. Graph details and symbols are explained in the legend to Fig. 4.