FIGURE 5.

Extracellular T cell-miRNA signature RT-qPCR plate to profile psoriasis patient sera. A, scheme of miRNA representation in the RT-qPCR custom plate used to profile serum circulating miRNome of psoriasis patients. B, unsupervised hierarchical clustering of log-transformed normalized relative quantities of either all detectable serum circulating miRNAs (upper panel) or the 26 miRNAs belonging to the in vitro CD4⁺ T cell-derived miRNA signature (lower panel). The distance was determined by Pearson correlation with average linkage. C, Ct values variability among 8 samples (four psoriasis patients and four healthy donors) for the exogenous spike-ins added before extraction (in blue: UniSp4 and UniSp5) and before retro-transcription (in green: UniSp6), miRNA global mean (red), and the endogenous normalization factor (in orange: miR-18a-5p).