TABLE 1.
Yeast strains and plasmids used in this study
| Strain/plasmid | Characteristics or genotype | Source |
|---|---|---|
| FGY3 (WT) | MAT α, ura3-52, lys2-801, ade2-101, trp1Δ1, his3Δ200, leu2Δ1 | Jiang et al. (50) |
| FGY2 (crd1Δ) | MAT α, ura3-52, lys2-801, ade2-101, trp1Δ1, his3Δ200, leu2Δ1, crd1Δ::URA3 | Jiang et al. (50) |
| FGY3 atg8Δ | Derivative of FGY3, atg8Δ::KanMX4 | This study |
| FGY2 atg8Δ | Derivative of FGY2, atg8Δ::KanMX4 | This study |
| FGY3 atg18Δ | Derivative of FGY3, atg18Δ::KanMX4 | This study |
| FGY2 atg18Δ | Derivative of FGY2, atg18Δ::KanMX4 | This study |
| FGY3 atg32Δ | Derivative of FGY3, atg32Δ::KanMX4 | This study |
| FGY2 atg32Δ | Derivative of FGY2, atg32Δ::KanMX4 | This study |
| FGY3 atg21Δ | Derivative of FGY3, atg21Δ::TRP1 | This study |
| FGY3 atg18Δatg21Δ | Derivative of FGY3, atg18Δ::KanMX4, atg21Δ::TRP1 | This study |
| FGY2 atg18Δatg21Δ | Derivative of FGY2, atg18Δ::KanMX4, atg21Δ::TRP1 | This study |
| FGY3-IDH1-GFP | Derivative of FGY3, in which GFP is Inserted into the IDH1 gene locus | This study |
| FGY2-IDH1-GFP | Derivative of FGY2, in which GFP is Inserted into the IDH1 gene locus | This study |
| BY4742 (WT) | MATα, his3Δ1, leu2Δ0, lys2Δ0, ura3Δ0 | Euroscarf a |
| VGY1 (BY4742 crd1Δ) | Derivative of BY4742, crd1Δ::URA3 | Gohil et al. (51) |
| BY4742 sho1Δ | Derivative of BY4742, sho1Δ:: KanMX4 | This study |
| VGY1sho1Δ | Derivative of BY4742, crd1Δ::URA3, sho1Δ::KanMX4 | This study |
| BY4742 ssk1Δ | Derivative of BY4742, ssk1Δ:: KanMX4 | This study |
| VGY1ssk1Δ | Derivative of BY4742, crd1Δ::URA3, ssk1Δ::KanMX4 | This study |
| BY4742 ste50Δ | Derivative of BY4742, ste50Δ::KanMX4 | This study |
| VGY1ste50Δ | Derivative of BY4742, crd1Δ::URA3, ste50Δ::KanMX4 | This study |
| BY4742 hog1Δ | Derivative of BY4742, hog1Δ::KAN | This study |
| VGY1hog1Δ | Derivative of BY4742, crd1Δ::URA3, hog1Δ::KanMX4 | This study |
| BY4742 WT-IDH1-GFP | Derivative of BY4742, in which IDH1 gene is chromosomally tagged with GFP | This study |
| BY4742 crd1Δ- IDH1-GFP | Derivative of BY4742 crd1Δ, in which IDH1 gene is chromosomally tagged with GFP | This study |
| pYPGK18 | 2 μm, LEU2 | Vaz et al. (52) |
| pYPGK18-PTP2 | Derivative of pYPGK18, expresses PTP2 from PGK1 promoter | This study |
| pYPGK18-PTP3 | Derivative of pYPGK18, expresses PTP3 from PGK1 promoter | This study |
| pYCp50 | CEN, URA3 | Helliwell et al. (53) |
| pYCp50-PKC1R398P | Derivative of pYCp50, expresses PKC1R398P from PGK1 promoter | Helliwell et al. (53) |
| pRS352 | 2 μm, URA3 | Helliwell et al. (53) |
| pRS352-BCK1-20 | Derivative of pRS316, expresses BCK1-20 from PGK1 promoter | Helliwell et al. (53) |
| pPS1739 | CEN, URA3, HOG1-GFP | Ferrigno et al. (54) |
| pFA6a-GFP(S65T)-His3MX6 | Derivative of pFA6a-His3MX6 | Kanki et al. (47) |
| pCu416-IDP1-GFP | Derivative of pCu416, expresses GFP-tagged IDP1 from CUP1 promoter | Journo et al. (55) |
a Euroscarf, European Saccharomyces Cerevisiae Archive for Functional analysis.