Figure 1. Induction of cytochrome P450 family 24 subfamily A member 1 (CYP24A1) by 25(OH)D₃ and 1α,25-(OH)₂D₃ in hMSCs.

Real-time quantitative PCR was used to determine the induction of CYP24A1 mRNA in response to 25(OH)D₃ (a) and 1α,25-(OH)₂D₃ (b) at the indicated concentrations in the hMSCs during three weeks of treatment. Relative mRNA expression was normalised to the control gene ribosomal protein lateral stalk subunit P0 (RPLP0) and fold inductions were calculated in reference to solvent control (0.1% ethanol), which is set as 1. Results are expressed as means ± SD (n = 3, ***P < 0.001).