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. 2017 Feb 17;7:42971. doi: 10.1038/srep42971

Figure 3. Absorption and fluorescence spectra of the W619_1-LOV protein reconstituted with flavin chromophores.

Figure 3

(a) Absorption spectra of W619_1-LOV bound to natural (FMN, RF, FAD) and structurally modified flavin chromophores (8-Cl-RF, 7-Br-RF, LC) (b) Absorption spectra of free chromophores used in panel a. (c) Fluorescence emission spectra of W619_1-LOV bound to natural (FMN, RF, FAD) and structurally modified flavin analogs (8-Cl-RF, 7-Br-RF, LC). (d) Fluorescence emission spectra of free chromophores used in panel c. Fluorescence emission measurements were done by excitation at a wavelength of 450 nm for all samples, except for LC where it was 420 nm.