Table 1. Photophysical and photochemical properties of W619_1-LOV bound to flavin chromophores.

Chromophore	Binding	Photocycle	Dark recoverya τ (min) at 20 °C	Absorbance λmax(nm)		Fluorescencebλmax (nm)		Quantum yieldb ΦF
				bound	free	bound	free	bound	free
FMN	yes	yes	460 ± 10	447	445	496	524	0.28	0.27
RF	yes	yes	1.5 ± 0.1	445	445	500	524	0.29	0.27
FAD	yes	yes	54.0 ± 0.5	447	450	499	523	0.25	0.03
7-Br-RF	yes	yes	28.5 ± 0.5	449	444	502	520	0.03	0.04
8-Cl-RF	yes	no	—	456, 474	442	510	518	0.10	0.02
LC	yes	no	—	337, 421	352, 387c	496	511	0.40	(n.d.)d

^aDark recovery was calculated by fitting data with exponential decay function , where t – time, A – absorbance at 474 nm and a₀, a₁ – scaling coefficients.

^bFluorescence spectra and quantum yield measurements were done by excitation at a wavelength of 450 nm for all samples, except for LC where it was 420 nm. Φ_F - relative quantum yield was calculated with RF as a reference (Φ_F = 0.27) according to formula , where A – absorbance at excitation wavelength, and I – integrated total fluorescence intensity.

^cLC shows two peak maxima.

^dNot determined as riboflavin is not a valid reference anymore.