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. 2017 Feb 17;7:42977. doi: 10.1038/srep42977

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Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Sections were prepared from a 163.7-mg seed. (a) Tandem mass spectrum (MS/MS) of the OPDA standard. The chemical formulae and structures are shown at the top of the respective peaks. (b) MS/MS spectrum of the immature seed section at m/z 290–293. Internal calibration of the MS/MS spectrum was performed post-acquisition using the exact m/z of OPDA [M-H]⁻ ion (m/z 291.1966). (c) Optical image of a seed section after DESI-IMS/MS. (d) Ion image at m/z 165.129. (e) Ion image at m/z 247.208. (f) Ion image at m/z 273.186. (g) Ion image at m/z 291.196. Scale bar = 2 mm.