Table 2. Abscisic acid (ABA) and 12-oxo-phytodienoic acid (OPDA) concentrations in the embryo and external structures of immature Phaseolus vulgaris L. seeds.
Tissue | Weight (mg FW) | OPDA (pg/mg FW) | ABA (pg/mg FW) |
---|---|---|---|
Embryo | 113 ± 7 | 53 ± 24 | 1844 ± 478 |
External structures | 59 ± 1 | 8164 ± 1549 | 103 ± 52 |
ABA and OPDA in aqueous methanol (80%) extracts of embryo and external structures separated from four different immature seeds were quantified by liquid chromatography-electrospray ionisation-tandem mass spectrometry (LC-ESI-MS/MS) analysis. Concentrations of ABA and OPDA in embryo and external structures are shown per mg of fresh weight (FW). Each value is represented by the mean ± standard deviation (n = 4).