Table 1. Detection of PLMVd in singly-inoculated and co-inoculated GF305 peach seedlings with two independent techniques.

Plant code	Variant inoculated	3 months p.i.			5 months p.i.
		rtRT-PCR		Gel-blot hybridization	rtRT-PCR			Gel-blot hybridization
		Probe P1	Probe P2		Probe P1	Probe P2	Probe P3
1	v1.1	−a	12.82b	+++c	−	12.06	12.99	+++
2	v1.1	−	13.13	+++	−	11.62	12.81	+++
3	v1.1	−	12.71	+++	−	10.62	12.97	+++
4	v1.1	−	14.66	−	−	12.92	14.11	+++
5	v1.1	−	27.52	−	−	12.13	13.79	+++
6	v1.1	−	12.56	+++	−	12.61	13.82	+++
7	v1.1	−	20.68	+++	−	12.04	12.97	+++
8	v1.1	31.21	27.53	+	−	12.03	12.83	+++
9	v1.1	−	12.67	+++	−	10.99	12.96	+++
10	v1.1	−	13.92	+	−	12.94	13.92	+++
11	v1.1 + gds6	−	25.14	−	−	12.16	12.96	+++
12	v1.1 + gds6	−	13.24	+++	−	11.80	13.53	+++
13	v1.1 + gds6	−	12.58	+++	34.15	11.30	14.09	+++
14	v1.1 + gds6	−	12.96	+++	−	12.10	13.59	+++
15	v1.1 + gds6	−	20.31	−	−	12.78	13.98	+++
16	v1.1 + gds6	−	12.79	+++	−	12.13	13.06	+++
17	v1.1 + gds6	−	12.72	+++	23.17	12.57	13.35	+++
18	v1.1 + gds6	−	18.59	−	31.52	12.68	13.90	+++
19	v1.1 + gds6	−	15.65	−	−	11.99	13.63	+++
20	v1.1 + gds6	−	14.77	−	24.22	13.13	13.46	+++
21	gds6	−	−	−	13.56	−	13.83	+++
22	gds6	−	−	−	15.55	−	16.67	+++
23	gds6	−	−	−	13.31	−	14.62	+++
24	gds6	15.18	−	+++	13.79	−	15.37	+++
25	gds6	29.53	−	−	16.31	−	17.67	+++
26	gds6	14.57	−	++	13.71	−	14.93	+++
27	gds6	31.87	−	−	16.21	−	16.91	+++
28	gds6	33.58	−	−	13.82	−	14.89	+++
29	gds6	32.79	−	−	13.58	−	14.44	+++
30	gds6	32.89	−	−	18.44	−	18.85	+++
31	Mock	−	−	−	−	−	34.67d	−
32	Mock	−	−	−	−	−	34.19d	−
33	Mock	−	−	−	−	−	35.72d	−

^aUndetected.

^bThreshold cycle (Ct).

^cSignal intensity from weak (+) to strong (+++).

^dThe high Ct values suggest a minor contamination during analysis because no signal was detected with probe P3 in non-inoculated controls of Prunus persica. P. dulcis. P. avium and P. armeniaca.