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. 2017 Feb 17;7:42725. doi: 10.1038/srep42725

Figure 3. Pro-fibrotic gene expression in TGF-β induced fibroblasts.

Figure 3

nCF transfected with scr siRNA or Fibulin-6 siRNA, after being exposed to TGF-β were subjected to RNA or protein extraction for real-time PCR or western blotting respectively. (a) Collagen I α1 and (b) CTGF mRNA expression is increased after TGF-β stimulation in scr- but not in fibulin-6-siRNA transfected nCF (n = 7, p < 0.05, nonparametric Mann-Whitney U test). Real-time RT-PCR signals were normalized to hypoxanthine phosphoribosyl-transferase (HPRT) gene expression and data are expressed as 2−ΔΔCT. (c and d) Densitometric analysis of western blots revealed upregulation of (c) collagen I α1 and (d) CTGF upon TGF-β stimulation in scr-siRNA transfected nCF. In fibulin-KD nCF no upregulation could be observed (n = 5, p < 0.05, nonparametric Mann-Whitney U test).