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(A) Arginase activity was evaluated, using a colorimetric assay, in the absence or presence of arginase inhibitor NOR-NOHA (n = 2; p = 0.02; error bars represent S.D.). (B–D) EV preparations (n = 3) characterized using cryo-EM (B), nanoparticle-tracking analysis (C) and immuno-EM with an antibody against Arg1 protein (D) Bar = 100 nm.
