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| Time | Ease of protocol | Clearing efficiency | GFP fluorescence preservation | 2-photon optical penetration | Nuclear size alteration | |
|---|---|---|---|---|---|---|
| RIMS | 1 day | Easy | Medium | yes | + | Decrease |
| SeeDB | 1 day | Easy | Weak | no | + | Not measured |
| 3DISCO | <1 day | Easy but manipulation of toxic compounds | Strong | no | + | High decrease |
| PACT | 13 days | Difficult | Strong | yes | +++ | Decrease |
| CUBIC | 5 days | Easy | Strong | yes | +++ | Increase |
