Figure 1.

(A) In vivo bioluminescence imaging of a representative athymic nude mouse 7 weeks after intracardiac inoculation of LADOB cells. Note the bioluminescence emanating from different locations from the spine, long bones, and the skull compatible with the multifocal skeletal pattern of metastases. (Middle) X-ray imaging of a femur showing the radio opacity in the two metaphyses spreading toward the diaphysis. (Right) Micro-CT scan of the same bone showing osteosclerotic lesions. (B, Left) H&E staining showing the presence of tumor cells surrounding dense patches of newly formed bone at the metaphyseal-diaphyseal location. (Middle) Immunostaining for pan-cytokeratin showing tumor cells surrounded with newly formed bone. (Right) TRAP staining showing positive immunoreactivity at the tumor-bone interphase.