Experimental design. (A) Five days after i.c. inoculation of A549M1 cells, animals were treated with a single dose of ZA (70 μg/kg) or vehicle. Ten mice for each treatment and each time point were sacrificed at 21 and 28 days. Bioluminescence was analyzed 7, 14, 21, and 28 days after A549 cells inoculation. (B) Five days after i.c. inoculation of LADOB cells, animals were treated with a single dose of ZA (70 μg/kg) or vehicle. Ten mice for each treatment and each time point were sacrificed at 60 and 75 days. Bioluminescence was analyzed 30, 45, 60, and 75 days after LADOB cells inoculation.