Fig. 6.
Putative plant heat responsive cis regulatory elements on E3900 (accession number AF222021). (A) The complete E3900 sequence is shown with the putative translation start site (AUG) and the 1132-bp deletion present in tE3900 shown by dashed line. The distribution of five cis elements on E3900 reveals two clusters approx. 1 kb and immediately upstream of the putative translation initiation site on the + strand of E3900. (B) Observed and expected frequencies of each cis regulatory element on the + and – strands of E3900 indicate that these are more frequent than expected on the + strand of the entire sequence (**χ2, P < 0·004), and not on the – strand (NSχ2 , P > 0·05). Strikingly, 18 of the 22 observed elements on the + strand are upstream of nucleotide 2024, which is highly significantly greater than expected (***χ2 , P < 4 × 10−7).