Figure 4.
Culture-expressed Bbive-TSSs demonstrate activity in the mammalian host. C3H/HeN mice infected intraperitoneally with 1 × 105B. burgdorferi containing promoterless pJSB161, control promoters (flaBp, ospCp or ospAp) or specific Bbive luciferase fusions as indicated (left). Mice were injected intraperitoneally with 150 mg/kg D-luciferin 7 and 10 days post infection and imaged with the IVISTM 50 Imaging System (IVIS) using a 5 min exposure. Images were normalized to the same p/s range of 1.73e4 to 1.128e5 and displayed on the same color spectrum scale (right). Data are representative of two independent biological replicates. asTSS, antisense TSS; p/iTSS, primary or internal TSS; iTSS, internal TSS.