Table 2. BbIVET-associated TSS read counts and luciferase promoter fusion relative activity.
| Promotera | Replicon | TSS Positionb | Strand | Categoryc | Category Details (Annotation)d | Avg. TAP+ Read Counte | AVG RLUf |
|---|---|---|---|---|---|---|---|
| flaBp | chr | 148715 | - | primary | P:BB_0147 (flagellin) | 9.39E+05 | 1.19E+06 |
| ospAp | lp54 | 9421 | + | primary | P:BB_A15 (ospA, outer surface protein A) | 1.94E+04 | 2.31E+05 |
| ospCp | cp26 | 16883 | + | primary | P:BB_B19 (outer surface protein C) | 37.5 | 134.4 |
| Bbive12a | chr | 174219 | - | internal, primary | I:BB_0172 (von Willebrand factor type A domain-containing protein), P:BB_0171 (hypothetical protein) | 1.42E+04 | 1.07E+04 |
| Bbive45 | chr | 379813 | - | internal | I:BB_0370 (tyrS, tyrosine–tRNA ligase) | 243 | 663.1 |
| Bbive74 | chr | 647833 | + | antisense | A:BB_0620 (beta-glucosidase) | 194 | 85.9 |
| Bbive76 | chr | 659711 | + | antisense | A:BB_0629 (PTS system fructose-specific transporter subunit IIABC) | 362 | 1.62E+03 |
| Bbive86 | chr | 746049 | + | internal, primary | I:BB_0709 (hypothetical protein), P:BB_0710 (dnaG, pseudo) | 1.58E+03 | 4.66E+03 |
| Bbive203 | chr | 146579 | - | internal, primary | I:BB_0146 (glycine/betaine ABC transporter ATP-binding protein), P:BB_0145 (glycine/betaine ABC transporter permease) | 6.71E+03 | 1.12E+04 |
aControl promoters and Borrelia burgdorferi in vivo expressed sequence identification numbers.
bReplicon specific nucleotide position of 5′RNA-seq identified TSS.
cTSS category assignment.
dAnnotation of the TSS associated ORF (50,90), where single letter abbreviations indicate the annotation for the TSS category.
eAverage normalized read counts from 5′RNA-seq TAP+ libraries, for a given TSS.
fPromoter activity for each TSS, as determined by luciferase activity in RLU, for spirochetes grown to log phase in liquid culture.