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. 2016 Aug 29;45(2):875–885. doi: 10.1093/nar/gkw747

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© The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 3. — Base-pair opening dynamics of WT and mutant pri-miR156a. (A) Hydrogen exchange rate constants (k_ex, s^–1) of the imino protons of WT (black), A9C (red), A9AC (purple), A10G (blue) and A10CG (green) pri-miRNA156a at 16°C (left) and 23°C (right). Asterisks indicate that the imino proton resonances exhibited severe line-broadening. (B) Hydrogen exchange times (τ_ex = 1/k_ex) for the G98 (upper) and U16 (lower) imino protons as a function of the Tris concentrations at 23°C. The solid lines are the best fits to Equation (6). (C) Equilibrium constants for base-pair opening (K_op), base-pair lifetimes (τ₀) and lifetimes for base-pair opening (τ_open) of WT (black), A9C (red), A9AC (purple), A10G (blue) and A10CG (green) pri-miRNA156a determined by the Tris-catalyzed NMR exchange experiments at 23°C.