. 2016 Aug 29;45(2):875–885. doi: 10.1093/nar/gkw747

Table 1. Hydrogen exchange rate constants (k_ex, s⁻¹) of the imino protons of the WT and mutant pri-miR156a constructs in 90% H₂O/ 10% D₂O buffer containing 10 mM TRIS-d₁₁, 50 mM NaCl at 16°C (pH = 8.25) and 23°C (pH = 8.03).

Temp	Imino	WT	A9C	A9AC	A10G	A10CG
16°C	G94	0.7 ± 0.4	0.7 ± 0.4	0.8 ± 0.4	0.9 ± 0.5	0.8 ± 0.4
	U95	3.8 ± 0.3	3.3 ± 0.2	3.4 ± 0.2	4.3 ± 0.3	3.4 ± 0.4
	G17	0.3 ± 0.2	0.4 ± 0.2	0.4 ± 0.2	0.3 ± 0.2	0.4 ± 0.3
	U16	7.5 ± 0.4	5.1 ± 0.4	5.9 ± 0.2	8.5 ± 0.4	4.2 ± 0.5
	G98	5.0 ± 0.6	3.8 ± 0.4	4.0 ± 0.3	4.4 ± 0.3	3.7 ± 0.5
	U100	n.d.^a	n.d.	198 ± 8	249 ± 64	194 ± 38
	U101	89.1 ± 4.9	78.5 ± 5.3	13.4 ± 0.4	17.3 ± 0.7	13.0 ± 0.8
	G11	13.2 ± 0.8	8.6 ± 0.7	4.9 ± 0.5	0.6 ± 0.4	0.9 ± 0.5
	G10	−^b	−	−	5.2 ± 0.7	1.0 ± 0.5
	G104	−	−	26.6 ± 0.9	−	12.9 ± 1.4
	G105	−	6.8 ± 0.6	−	−	−
23°C	G94	1.0 ± 0.2	1.0 ± 0.4	1.4 ± 0.4	1.5 ± 1.0	0.9 ± 0.4^d
	U95	4.8 ± 0.3	4.5 ± 0.6	7.2 ± 0.5	8.9 ± 0.9	5.8 ± 0.7
	G17	0.4 ± 0.2	0.5 ± 0.2	0.4 ± 0.3	0.5 ± 0.4	0.5 ± 0.2
	U16	11.1 ± 0.4	7.8 ± 0.7	13.5 ± 0.6	19.0 ± 0.9	6.6 ± 0.9
	G98	7.2 ± 0.5	5.4 ± 0.8	8.7 ± 0.5	10.0 ± 0.9	6.7 ± 0.9
	U100	n.a.^c	n.a.	n.d.	n.d.	n.d.
	U101	n.d.	n.d.	39.8 ± 1.7	43.5 ± 1.2	26.7 ± 1.2
	G11	32.5 ± 3.4	17.8 ± 2.8	11.1 ± 1.2	1.2 ± 0.7	1.0 ± 0.6
	G10	−	−	−	11.2 ± 0.8	0.9 ± 0.4^d
	G104	−	−	86.8 ± 12.4	−	16.1 ± 1.6
	G105	−	15.0 ± 1.4	−	−	−

^aNot determined because of severe line-broadening.

^bNo imino proton resonance.

^cNot available because the imino proton resonance disappeared.

^dThe G10 and G94 resonances overlapped with each other.

Table 1. Hydrogen exchange rate constants (kex, s−1) of the imino protons of the WT and mutant pri-miR156a constructs in 90% H2O/ 10% D2O buffer containing 10 mM TRIS-d11, 50 mM NaCl at 16°C (pH = 8.25) and 23°C (pH = 8.03).