Figure 6.

RAD51 nucleoprotein filament is not stabilized by BCCIPβ. (A) Schematic of the nuclease protection assay (adapted from Chi et al. 2007 (41)). (B) RAD51 (0.4 μM) was incubated with radiolabeled ssDNA (3 μM nucleotides) alone (lane 3) or with increasing concentrations of BCCIPβ (lanes 4–6; 0.4 μM, 0.8 μM, 1.2 μM, respectively). The reaction was initiated by the addition of DNase I (2 units). Reaction products were then deproteinized and separated using 10% polyacrylamide gel electrophoresis. Lane 1 contained radiolabeled ssDNA alone, lane 2 contained only radiolabeled ssDNA in the presence of DNase I, and lane 7 contained BCCIPβ (1.6 μM) in the absence of RAD51 but in the presence of DNase I. deg, degraded radiolabeled ssDNA.