Figure 7.

Interaction with BCCIPβ induces conformational changes in RAD51. (A) RAD51 (5 μM) was incubated with trypsin (20 μg/ml) in the presence and absence of ATP (2 mM), ϕX174 ssDNA (30 μM nucleotides), calcium (1.8 mM) and BCCIPβ (10 μM), as indicated. The reactions were stopped with SDS and heat. The reaction products were resolved using SDS-PAGE followed by western blot analysis. Antibodies against RAD51 were used to develop the membrane. (B) The amounts of each band from undigested RAD51 and Fragments A, B, C and D were graphed based on the relative intensity of each band. Quantitation of the proteolytic fragmentation of RAD51 was determined from three independent experiments.