Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Sep 30;45(2):711–725. doi: 10.1093/nar/gkw877

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

PMC Copyright notice

Figure 8. — BCCIPβ stimulates RAD51 ATP hydrolysis and promotes ADP release. (A) RAD51 (0.5 μM) ATP hydrolysis assay in the presence or absence of ϕX174 ssDNA (60 μM nucleotides) and BCCIPβ (1 μM). (B) Time course analysis of RAD51 (0.5 μM) ATP hydrolysis in the presence of ϕX174 ssDNA (60 μM nucleotides), with or without BCCIPβ (1 μM). Error bars represent s.e.m. (n = 3); P-value *< 0.05. (C) Fluorescence spectra (400–500 nm) of MANT-ADP (0.5 μM) and ϕX174 ssDNA (2.0 μM nucleotides) in the absence or presence of RAD51 or RAD51_K133A (0.5 μM). (D) Time course analysis of MANT-ADP dissociation from a RAD51-MANT-ADP-ssDNA complex in the presence of magnesium with or without BCCIPβ (1 μM). (E) Time course analysis of MANT-ADP release from the RAD51-MANT-ADP-ssDNA filament in the presence of calcium with or without BCCIPβ (1 μM) and/or ATP (5 μM), as indicated. P-value *< 0.05 and **< 0.01 is indicative of the 600 s time point.