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. 2015 Jun 10;3(1):e1056905. doi: 10.1080/21690707.2015.1056905

Figure 5.

Figure 5.

pH Dependence of Spherulite Growth (A) Far UV circular dichroism (CD) spectra of Alamanda PLE (7.5 kDa, 1 mg/mL, 37 °C) as function of solution pH. Alamanda PLE undergoes the typical cooperative transition from an alpha-helix to a random-coil conformation between pH 4.5 and 5.5.67,58 (B) Slow down in the kinetics of PLE spherulite growth as pH approaches the edge for the alpha-helix conformation (C, D) ThT fluorescence responses from 1 mg/mL solutions of 7.5 kDa PLE incubated at high salt concentrations either (C) in the random coil state (pH 6.0) or (D) near the edge of the alpha helical conformation (pH 4.5).