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Temperature Effects on Monomer Structure and Spherulite Growth (A) Self-assembly of PLE (0.5 mg/mL, pH 3.6, 50 mM NaCI) into spherulites monitored at three different temperatures using ThT (10 [μM) as indicator dye. (B) CD spectra of PLE (0.2 mg/mL, pH = 3.6) vs. temperature. CD spectra indicate a gradual transition from α-helix to random coil conformation as temperature increases.
