FIG. 3.

Evaluation of bone microstructure in the TE bone. (A) Representative three-dimensional architecture of engineered bone made with osteoblasts only (hOB) or both cell types (hOB + hOC) obtained by μCT imaging (n = 3). (B) Plots of BV/TV and Conn D) from μCT images of the two experimental groups (n = 3). (C) Immunohistochemical staining of BSP in engineered bone made with osteoblasts only (hOB) or both bone cell types (osteoblasts and osteoclasts; hOB + hOC) untreated and treated with ZA. Magnified views are shown in the bottom panels. μCT, microcomputed tomography; BV/TV, bone volume fraction; Conn D, connectivity density; ZA, zoledronic acid. Color images available online at www.liebertpub.com/tec