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. 2017 Feb 1;28(1):49–59. doi: 10.1089/hgtb.2016.089

Figure 1.

Figure 1.

Y447F+Y733F-AAV8 double mutant improves transduction efficiency in mice pancreas. (A) Ex vivo tissue fluorescence imaging and quantitative analysis of mCherry expression following i.p. injection of AAV8 WT, 1M, 2M, and 3M. Representative image is shown (left panel). Scale bars presented as Radiant Efficiency [(p/s/cm2/sr)/(μW/cm2)]. Results are shown as the mean ± SE (right panel) (*p < 0.1, n = 3 per group). (B) Fluorescence image and quantification of percent mCherry expression efficiency in pancreas and liver tissue. Representative images are shown. Blue: DAPI staining for nuclei; red: mCherry expression. Scale bar: 65 μm. Results in the lower panel are shown as the mean ± SE (*p < 0.1***, p < 0.001, n = 3 per group, two sections from each animal were analyzed). (C) Quantitative real-time PCR analysis of AAV genomes. Results are shown as the average AAV genome copy number per microgram of total DNA ± SE (*p < 0.1, n = 3 per group). AAV, adeno-associated virus; F, phenylalanine; i.p., intraperitoneal; M, mutant; SE, standard error; WT, wild type; Y, tyrosine.