Figure 3.
Y447F+Y733F-AAV8 efficiently transduces mouse pancreatic ductal adenocarcinomas in KrasG12D+/− and KrasG12D/+/PTEN+/− genetically engineered mice. (A) Haemotoxylin and eosin staining of mouse PDAC. (B) Expression of mCherry in PDAC under fluorescence microscopy. Blue: DAPI staining for nucleus; red: mCherry expression. Scale bar: 65 μm. (C) Quantification of percent mCherry expression efficiency in PDAC. Results are shown as the mean ± SE (**p < 0.01, n = 2 per group, three sections from each animal were analyzed). (D) Quantitative analysis of viral genome copy number by real-time PCR. Results are shown as the mean ± SE (n = 2 per group). PDAC, pancreatic ductal adenocarcinoma.