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. 2017 Feb 1;28(1):39–48. doi: 10.1089/hgtb.2016.180

Figure 1.

Figure 1.

Adeno-associated virus (AAV) stability at 4°C and after freeze–thaw cycles. (a) AAV1-CaMKII-eNpHR3.0-iRFP was diluted and stored at 4°C for up to 7 weeks. Rat primary cortical neurons were transduced on DIV6 with AAV and imaged on day in vitro (DIV) 15. Graphs show each sample normalized to control with mean and standard deviation (SD). # indicates significantly different from the freshly thawed group (0 weeks at 4°C), p < 0.05; three independent experiments, three samples per experiment). * indicates significantly different from all others in group, p < 0.05). (b) AAV1-CaMKII-eNpHR3.0-iRFP was frozen and thawed up to 10 times. Rat primary cortical neurons were transduced on DIV 6 and imaged on DIV 15. Graphs show each sample normalized to control with mean and SD. No significant difference among groups (three independent experiments, two to three samples per experiment).