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. Author manuscript; available in PMC: 2017 Apr 15.
Published in final edited form as: J Immunol. 2016 Mar 7;196(8):3411–3420. doi: 10.4049/jimmunol.1500906

Figure 1.

Figure 1

Inhibiting IL-1β protects fetal airway development from the effects of LPS. E15 fetal lung explants from C57BL/6 mice were cultured in the presence of LPS (250 ng/ml) along with (A) nonimmune IgG, (B) neutralizing antibodies against IL-1β (10 ng/ml), (C) neutralizing antibodies against TNFα (10 ng/ml), or (D) both IL-1β and TNFα antibodies. Brightfield images from representative explants illustrate airway formation between 24–72 h of culture. (E). LPS inhibits airway formation, but antibodies against IL-1β prevented this inhibition (*P < 0.05, n = 18–53). Anti-TNFα had no effect. (F). Antibodies against IL-1β and TNFα did not prevent the initial inflammatory response, as measured by Ccl2 expression (*P < 0.05, n = 5).