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. Author manuscript; available in PMC: 2017 Apr 15.
Published in final edited form as: J Immunol. 2016 Mar 7;196(8):3411–3420. doi: 10.4049/jimmunol.1500906

Figure 5.

Figure 5

Changes in inflammasome expression throughout lung development. (A–B). Gene expression of inflammasome substrates and components in E12, E13, E15, E17, P1, and adult lung. (*P < 0.05, n = 4.) Gapdh expression used as internal control. Values normalized to E12 samples and expressed as fold change using the 2−ΔΔCT formula. (C). Immunoblotting of lung homogenates failed to detect NLRP1 protein expression before E18. NLRP1 expression was highly abundant in adult lung samples (lighter exposure of adult lung homogenate shown at right). ASC protein expression was detected by immunoblotting from E13-E18. GAPDH expression was measured as a protein loading control.