Table 1. List of antibodies used in the experiments.
| Clone | Recognized epitop | Fluorochrome conjugated | Application | Supplier | ||
|---|---|---|---|---|---|---|
| Anti-MDR1/ABCB1 | MDR1/1 | 15D3 | external | PE | FC | BD Pharmingen |
| MDR1/2 | 17F9 | external | PE | FC | BD Pharmingen | |
| MDR1/3 | UIC-2 | external | FC | IITD | ||
| MDR1/4 | E1Y7S | WB, ICC | Cell Signaling | |||
| MDR1/5 | polyclonal | WB | ThermoFisher Scientific | |||
| Anti-MRP1/ABCC1 | MRP1/1 | QCRL-2 | internal | FITC | FC | Santa Cruz |
| MRP1/2 | QCRL-3 | internal | FC | IITD | ||
| MRP1/3 | polyclonal | WB, ICC | ThermoFisher Scientific | |||
| MRP1/4 | polyclonal | WB | Cell Signaling | |||
| MRP1/5 | MRPm6 | WB | Alexis Biochemicals | |||
| Anti-MRP4/ABCC4 | MRP4/1 | M4I-80 | WB, ICC, FC | LifeSpan BioScience | ||
| MRP4/2 | D1Z3W | WB, ICC, FC | Cell Signaling | |||
| Anti-MRP5/ABCC5 | MRP5/1 | M5I-10 | WB, ICC, FC | Kamiya | ||
| Anti-BCRP/ABCG2 | BCRP/1 | 5D3 | external | PE | FC | BD Pharmingen |
| BCRP/2 | BXP-21 | internal | WB, ICC, FC | EnzoLife Sciences | ||
| BCRP/3 | polyclonal | WB | Cell Signalling | |||
| BCRP/4 | BXP-34 | internal | WB, ICC | Alexis Biochemicals | ||
| Anti-B-actin | B-actin | D6A8 | WB | Cell Signaling |
FC—flow cytometry, WB—Western blotting, ICC—immunocytochemistry. Antibodies used for FC were unconjugated or directly conjugated to fluorochrome: fluorescein isothiocyanate (FITC) or phycoerythrin (PE). Antibodies MDR1/3 and MRP1/2 were prepared by ourselves at the Institute of Immunology and Experimental Therapy (IITD PAN).